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  6.  » Catalent’s Advanced Bioassay for Evaluating Cell Response

Catalent’s Advanced Bioassay for Evaluating Cell Response

Summary:

Understanding transcriptional activity within cells is crucial for evaluating responses to ligands or promoter activity in transgenes or plasmids. Catalent has pioneered a relative potency bioassay using real-time quantitative reverse transcription (RT-qPCR) in a duplex format to measure transcription activity in cells treated with ligands or transgenic vectors.

Key Features:

  • Duplex Format: Utilizes two fluorescent dyes with minimally overlapping emission spectra for real-time monitoring of both target and normalizer gene expression.
  • Simplified Process: Eliminates the need for mRNA purification, enhancing efficiency and accuracy.
  • Normalized Data: Normalizes qPCR cycle thresholds (CT) of the target transcript to the reference transcript, generating a response curve for comparison to a reference standard.
  • Four-Parameter Fit Curve Analysis: Allows comparison of relative potency and assessment of suitability based on curve parallelism.

Catalent’s assay platform is reliable, accurate, and specific, offering a linear response and reproducibility. This makes it an invaluable tool for evaluating the relative potency of various test substances.

Join us to discover how these robust cell-based potency assays can enhance your research and provide critical data on drug product potency.

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