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Case Study for Evaluation of the 3M™ Emphaze™ AEX Hybrid Purifier

Summary: The 3M™ Emphaze™ AEX Hybrid Purifier is a novel purification tool that utilizes a Q-functional hydrogel and 0.2 μm membrane to reduce impurities in a biopharmaceutical downstream process. This study involved a Design of Experiment (DoE) basedapproach to investigate the effects of pH and conductivity on the turbidity, yield, and impurity clearance of the Emphaze™ AEX Hybrid Purifier and the efficiency of the subsequent protein capture step. Filtration using the Emphaze™ AEX Hybrid Purifier resulted in a greater host cell protein (HCP) reduction and lower turbidity than unfiltered pools.

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