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In-Line Cell Lysis

Summary:​ Cell lysis is one of the most critical steps during plasmid DNA (pDNA) bioproduction for cell and gene therapy applications. The cell lysis step aims at recovering the plasmid material contained within the E. coli cells stemming from the fermentation unit operation, and to separate this plasmid material from the rest of the cell components. Here we present an in-line cell lysis assembly developed at Catalent to increase throughput while controlling critical parameters such as time of contact and solutions ratio, delivering a high-quality product.

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