Integrating DNA Sequencing and AI for AAV Genome Integrity
Summary: AAV genomic integrity is based on defining the molecular DNA composition of recombinant AAV vector preparations, specifically, the presence of full-length, ITR-to-ITR genomes, and the relative amount of undesirable molecular sub-species encapsidated within AAV particles. Variation in the profile of the intact AAV vector genome leads to product heterogeneity and contaminants that might impact safe and effective biological outcomes. This webinar explores the role of long-read sequencing in assessing genome integrity. Characterizing the molecular profile may provide insights that could inform more effective vector design early in the research phase, shorten development timelines, and reduce cost-of-goods.