Radiance® Label-Free Monitoring of AAV Transfection in HEK293 Cells Using Laser Force Cytology™
Summary: Along with the complexities of gene therapy process development comes the need for analytical techniques that can help assess quality parameters. In this technical note, Catalent and LumaCyte experts collaborate to compare AAV production with three different transfection reagents using both LumaCyte’s Laser Force Cytology (LFC) instrument, Radiance®, and a digital droplet PCR (ddPCR) based viral genome titer assay.