Home » Solutions » Development » Bioconjugate Development » Publications

Antibody Drug Conjugates And Bioconjugates


Drake PM et al.  CAT-02-106, a site-specifically conjugated anti-CD22 antibody bearing an MDR1-resistant maytansine payload yields excellent efficacy and safety in preclinical models.   Mol Cancer Ther. 2018,17: 161-168.

Barfield RM, Rabuka D.  Leveraging Formylglycine Generating Enzyme for Production of Site-Specifically Modified Bioconjugates.  Methods Mol Biol Noncanonical Amino Acids.  Ed. Lemke EA. 2018, 3-16.

Holder PG, Rabuka D. Technologies for Antibody Drug Conjugation.  Biosimilars of Monoclonal Antibodies:   A Practical Guide to Manufacturing and Preclinical and Clinical Development.  Ed. Cheng Liu 2017.

Albers A et al.  A Stable, versatile conjugation chemistries for modifying aldehyde-containing biomolecules.  Chemical Ligation: Tools for Biomolecule Synthesis and Modification.  Ed. D’ Andrea, Romanelli A. 2017.

Botzanowski T et al. Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates. MAbs20179:801-811.

Walder R et al. Rapid Characterization of a Mechanically Labile a-helical Protein enabled by Efficient Site-Specific Bioconjugation. J Am Chem Soc. 2017,139:9867-9875.

Drake PM, Rabuka D. Recent Developments in ADC Technology: Preclinical Studies Signal Future Clinical Trends.  Biodrugs20176:521-531.

Zmolek W et al. A simple LC/MRM-MS-based method to quantify free linker-payload in antibody-drug conjugate preparations. Chromatogr B Analyt Technol Biomed Life Sci. 2016 Jun 1.

Bleck G et al.  Generating a Fully Processed Antibody.  Biopharm International. 2016, Aug  40-42.

Kudirka RA et al.  Site-Specific Tandem Knoevenagel Condensation-Michael Addition to Generate Antibody-Drug Conjugates.  ACS Med Chem Lett. 2016, 7:994.

York D et al. Generating aldehyde-tagged antibodies with high titers and high formylglcyine yields by supplementing culture media with copper(II). BMC Biotechnology. 2016, 16(1):23.


Kudirka R et al.  Generating Site-Specifically Modified Proteins via a Versatile and Stable Nucleophilic Carbon Ligation.  Chemistry & Biology 2015, 22:1-6.

Barfield RM et al. Designing Optimized, Site-Specific ADCs.  Manufacturing Chemist Pharma. 2015, 86:38-41.

Holder PG et al.  Reconstitution of Formylglycine-generating Enzyme with Copper(II) for Aldehyde Tag Conversion.  J. Biol. Chem. 2015, 290:15730-45.

Kudirka R, Rabuka D.  The Hydrazino-iso-Pictet-Spengler Ligation: a Versatile, Mild, and Efficient Aldehyde Conjugation Strategy to Generate Site-Specific, Positionally Programmable Antibody-Drug   Conjugates.  American Pharmaceutical Review2015, June 11.

Drake PM and Rabuka D. An emerging playbook for antibody-drug conjugates: lessons from the laboratory and clinic suggest a strategy for improving efficacy and safety.  Curr Opin Chem Biol. 2015, 28:174-80.

Albers AE et al.  Exploring the effects of linker composition on site-specifically modified antibody-drug conjugates. Eur J Med Chem.  2014, 88:3-9.

Bhat AS et al.  The Next Step in Homogeneous Bioconjugate Development. Bioprocess International  2014, 12:Supplement 10-17.

Liang SI et al.,  A Modular Approach for Assembling Aldehyde-tagged Proteins on DNA Scaffolds.  J Am Chem Soc. 2014, 136:10850-3.

Drake PM et al.,  Aldehyde Tag Coupled with HIPS Chemistry Enables the Production of ADCs Conjugated Site-Specifically to Different Antibody Regions with Distinct in Vivo Efficacy and PK Outcomes.  Bioconjugate Chem., 2014, 25:1331-41.

Barfield RM, Rabuka D. ADC Development Using SMARTagTM Technology.  Drug Dev. and D.  2014, 14:34-41.

Drake PM, Rabuka D. Antibody-Drug Conjugates: Can Coupling Cytotoxicity and Specificity Overcome Therapeutic Resistance? Resistance to Immunotherapeutic Antibodies in Cancer Ed. Ben Bonavida 2013.

Agarwal PW et al., HydrazinoPictet-Spengler Ligation as a Biocompatible Method for the Generation of Stable Protein Conjugates. Bioconjugate Chem., 2013, 24: 846–851.

Agarwal PW et al., Pictet-Spengler ligation for protein chemical Modification. Proc Natl Acad Sci USA., 2013, 110: 46-51.

Rabuka D et al.  Site-specific Chemical Protein Conjugation Using Genetically Encoded Aldehyde Tags. Nat. Protoc. 2012 7:1052-67.

Hudak JE et al., Synthesis of heterobifunctional protein fusions using copper-free click chemistry and the aldehyde tag. Angew Chem Int Ed Engl. 2012: 4161-5.

Rabuka D et al., Site-specific chemical protein conjugation using genetically encoded aldehyde tags. Nat Protoc. 2012 7:1052-67.

Rabuka D, Chemoenzymatic methods for site-specific protein modification. Curr Opin Chem Biol. 2011 14: 790-6.

Rabuka D Chemoenzymatic methods for site-specific protein modification. Curr Opin Chem Biol 2010 14:790-6.

Wu P et al., Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag. Proc Natl Acad Sci U S A, 2009. 106: 3000-5.

Carrico IS et al, Introducing genetically encoded aldehydes into proteins. Nat Chem Biol, 2007. 3: 321-2.

Huang BCB et al. Antibody-drug conjugate library prepared by scanning insertion of the aldehyde tag into IgG1 constant regions. mAbs. 2018.